DAB substrate kit for detection of peroxidase (HRP) activity on immunoblots, immunohisto- and cytochemical slides and during in situ hybridization.

ROTI^®DAB Kit contains substrate, buffer solution and activation reagent, therefore including all three solutions necessary for HRP detection reaction. Application is very simple - all three kit solutions are diluted equally in water, and the blotting filter membrane, or the slides, are then incubated in this substrate solution. Since the solutions are delivered in drop dispensers, dosing is most convenient.

DAB is oxidized by HRP, forming a brown precipitate, which is insoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage.

Addition of the DAB Metal Enhancer (Art. No. 9204) doubles the signal strength. Slides may be permanently mounted in hydrophilic, or, after dehydration, in hydrophobic mounting medium.

DAB solution (Art. No. 9869), DAB buffer (Art. No. 9870), and Activation reagent (Art. No. 9871) in dropper bottles. Contents of this Kit may not be bought separately.