Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family. Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.

Foreign activity: RNAse and DNAse not detectableOptimum temperature: +65 °C.Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.Activators: Denaturating agents like SDS (0,5-1 %), urea.

A non-specific endopeptidase with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.

Working solution: 50 µg/mlReaction buffer:50 mM Tris-HCl; pH 7.5; 5 mM CaCl₂; 0.5 % SDS