Leibovitz’s L15 Medium was developed for culturing of eukaryotic cells without CO₂ enriched atmosphere. Its unique components include the use of galactose instead of glucose, omittance of bicarbonate, addition of free base forms of arginine, cysteine, and histidine, as well as elevated levels of tyrosine. Leibovitz’s L15 Medium is still used to grow cell lines in the absence of CO₂.

Generally, FBS is added to Leibovitz’s L15 media.

Leibovitz’s L15 Medium contains D-galactose instead of D-glucose and a high pyruvate concentration, but no sodium bicarbonate.